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fibroblast growth factor 2  (R&D Systems)


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    R&D Systems fibroblast growth factor 2
    Fibroblast Growth Factor 2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 908 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+fgf+basic/Recombinant+Human+FGF+basic%2FFGF2%2FbFGF+(146+aa)+Protein/pm42822105-87-45-50
    Average 96 stars, based on 908 article reviews
    fibroblast growth factor 2 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Transient proliferation by reversible YAP-mediated increase of the cyclin D1/p27 ratio
    Article Snippet: RPE1 cells were transfected with siRNA and sgRNA using DharmaFECT 1 (1:500 in Opti-MEM, Dharmacon Cat# T-2001-03) according to manufacturer protocol using 20 nM siRNA, or sgRNA and tracrRNA (in 1:1 ratio, according to manufacturer protocol).

    Passaging:

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations.
    Article Snippet: E14Ju09 ES cells [88] were routinely maintained in FCS (Gibco) with 100u/ml LIF (produced in-house) on gelatinized plates as described in [82]. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%– 80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [49,50]. hESCs were plated at 10,000 cells/cm2 on Geltrex in N2B27 media supplemented with Y-27632 10uM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations
    Article Snippet: MasterShef7 hESCs were cultured on Geltrex (Gibco) coated wells in mTeSR Plus media. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%–80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [ , ]. hESCs were plated at 10,000 cells/cm 2 on Geltrex in N2B27 media supplemented with Y-27632 10μM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Concentration Assay:

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations.
    Article Snippet: E14Ju09 ES cells [88] were routinely maintained in FCS (Gibco) with 100u/ml LIF (produced in-house) on gelatinized plates as described in [82]. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%– 80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [49,50]. hESCs were plated at 10,000 cells/cm2 on Geltrex in N2B27 media supplemented with Y-27632 10uM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations
    Article Snippet: MasterShef7 hESCs were cultured on Geltrex (Gibco) coated wells in mTeSR Plus media. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%–80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [ , ]. hESCs were plated at 10,000 cells/cm 2 on Geltrex in N2B27 media supplemented with Y-27632 10μM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Recombinant:

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations.
    Article Snippet: E14Ju09 ES cells [88] were routinely maintained in FCS (Gibco) with 100u/ml LIF (produced in-house) on gelatinized plates as described in [82]. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%– 80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [49,50]. hESCs were plated at 10,000 cells/cm2 on Geltrex in N2B27 media supplemented with Y-27632 10uM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Article Title: Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratio.
    Article Snippet: .. Human growth factors and mitogens were dissolved in sterile, ultrapure water (Fisher Scientific Cat#10977023) and used at the following concentrations unless otherwise stated: 25 ng/mL recombinant EGF (PeproTech cat#AF-100-15), 25 ng/mL recombinant HGF (Life Technologies Cat#PHG0254), 25 ng/mL recombinant FGF basic (R&D Systems Cat233-FB-025), 1 μM LPA (oleoyl-L-alpha-lysophosphatidic acid, Sigma-Aldrich Cat#L7260-1MG). .. Drugs were dissolved in DMSO (Sigma-Aldrich Cat# D2650) and used at the following concentrations unless otherwise stated: 0.5 μM TDI-011536 (Selleck Chemicals Cat#E1314, CAS#2687970-96-1, LATSi), 5 μM TRULI (a gift from James Hudspeth, CAS#1424635-83-5), 1 μM GNE-7883 (ChemieTek Cat#CT-GNE7883, CAS# 2648450-42-2,TEADi), 10 μM gefitinib (Selleck Chemicals Cat#S1025, CAS#184475-35-2, EGFRi), 0-20 μM erlotinib (Selleck Chemicals Cat#S1023, CAS#183321-74-6, EGFRi), 0-20 μM bemcentinib (MedChem Express Cat#HY-15150, CAS#1037624-75-1, AXLi), 0-20 μM RMC-4550 (MedChem Express Cat#HY116009, CAS#2172651-73-7, SHP2i), 0-20 μM BI-2865 (MedChem Express Cat#HY-153724, CAS#2937327-93-8, RASi), 10 μM LY294002 (Cayman Chemical Cat#70920, CAS#154447-36- AR TI CL E IN P RE SS 6 , PI3Ki), 200 nM MK2206 (Cayman Chemical Cat#11593, CAS#1032350-13-2, AKTi), 100 nM Torin2 (Cayman Chemical Cat#14185, CAS#1223001-51-1, mTORi), 100 nM PD0325901 (Selleck Chemicals Cat#S1036, CAS#391210-10-9, MEKi), 1 μM palbociclib (PD-0332991, Selleck Chemicals cat#S1116, CAS#827022-32-2, CDK4/6i), 0.5 μg/mL doxycycline hyclate (Sigma-Aldrich cat#D9891, CAS#24390-14-5, DOX), 10 μM 5-ethynyl-2’-deoxyuridine (EdU, Cayman Chemical Cat#20518, CAS# 61135-33-9).

    Article Title: A toolkit for mapping cell identities in relation to neighbors reveals conserved patterning of neuromesodermal progenitor populations
    Article Snippet: MasterShef7 hESCs were cultured on Geltrex (Gibco) coated wells in mTeSR Plus media. .. Routine hESCs passaging was performed with accutase in a 1:10 ratio once 70%–80% hESC confluency was reached, typically every 4–5 days. mTeSR Plus media was supplemented with ROCK inhibitor Y-27632 10μM for the first 24 h after passaging. hESCs were differentiated to hNMP-LCs as described in [ , ]. hESCs were plated at 10,000 cells/cm 2 on Geltrex in N2B27 media supplemented with Y-27632 10μM with the desired concentration of recombinant FGF basic (R&D systems) and CHIR99021 (Axon). ..

    Sterility:

    Article Title: Transient proliferation by reversible YAP and mitogen control of the cyclin D1/p27 ratio.
    Article Snippet: .. Human growth factors and mitogens were dissolved in sterile, ultrapure water (Fisher Scientific Cat#10977023) and used at the following concentrations unless otherwise stated: 25 ng/mL recombinant EGF (PeproTech cat#AF-100-15), 25 ng/mL recombinant HGF (Life Technologies Cat#PHG0254), 25 ng/mL recombinant FGF basic (R&D Systems Cat233-FB-025), 1 μM LPA (oleoyl-L-alpha-lysophosphatidic acid, Sigma-Aldrich Cat#L7260-1MG). .. Drugs were dissolved in DMSO (Sigma-Aldrich Cat# D2650) and used at the following concentrations unless otherwise stated: 0.5 μM TDI-011536 (Selleck Chemicals Cat#E1314, CAS#2687970-96-1, LATSi), 5 μM TRULI (a gift from James Hudspeth, CAS#1424635-83-5), 1 μM GNE-7883 (ChemieTek Cat#CT-GNE7883, CAS# 2648450-42-2,TEADi), 10 μM gefitinib (Selleck Chemicals Cat#S1025, CAS#184475-35-2, EGFRi), 0-20 μM erlotinib (Selleck Chemicals Cat#S1023, CAS#183321-74-6, EGFRi), 0-20 μM bemcentinib (MedChem Express Cat#HY-15150, CAS#1037624-75-1, AXLi), 0-20 μM RMC-4550 (MedChem Express Cat#HY116009, CAS#2172651-73-7, SHP2i), 0-20 μM BI-2865 (MedChem Express Cat#HY-153724, CAS#2937327-93-8, RASi), 10 μM LY294002 (Cayman Chemical Cat#70920, CAS#154447-36- AR TI CL E IN P RE SS 6 , PI3Ki), 200 nM MK2206 (Cayman Chemical Cat#11593, CAS#1032350-13-2, AKTi), 100 nM Torin2 (Cayman Chemical Cat#14185, CAS#1223001-51-1, mTORi), 100 nM PD0325901 (Selleck Chemicals Cat#S1036, CAS#391210-10-9, MEKi), 1 μM palbociclib (PD-0332991, Selleck Chemicals cat#S1116, CAS#827022-32-2, CDK4/6i), 0.5 μg/mL doxycycline hyclate (Sigma-Aldrich cat#D9891, CAS#24390-14-5, DOX), 10 μM 5-ethynyl-2’-deoxyuridine (EdU, Cayman Chemical Cat#20518, CAS# 61135-33-9).



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